G5635

Sigma

 

β-Galactosidase from Escherichia coli

Grade VIII, lyophilized powder, 600-1,200 units/mg protein

Synonym:β-D-Galactoside galactohydrolase, Lactase
CAS Number:9031-11-2
Enzyme Commission (EC) Number:3.2.1.23   ( BRENDA | IUBMB )
EC Number:232-864-1
MDL number:MFCD00130623

Description

ApplicationMay be used as a positive control protein with anti-β-galactosidase antibodies.
Unit DefinitionOne unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
Physical formContains Tris buffer salts and magnesium chloride
Physical propertiesTetramer molecular weight 465 kDa (subunits 116.3 kDa each)

Properties

typeGrade VIII
formlyophilized powder
does not contain BSA as standardization of activity
compositionProtein, ≥70% biuret
EQP levelPremium
storage temp.−20°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany3
RTECSLW5840000

Related Products

SubstrateF2756, Fluorescein di(β-D-galactopyranoside)
 C1546, 2-Chloro-4-nitrophenyl β-D-galactopyranoside
 N9643, Naphthofluorescein di-(β-D-galactopyranoside)
 M1633, 4-Methylumbelliferyl β-D-galactopyranoside
 B4252, 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside
 B4387, 5-Bromo-3-indolyl β-D-galactopyranoside
 B6024, 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside
InhibitorD6518, Diethylenetriaminepentaacetic acid
 E4378, Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid
 P9375, 1,10-Phenanthroline monohydrate
 ED2SS, Ethylenediaminetetraacetic acid disodium salt dihydrate

References

referenceFowler, A.V., et al., Amino acid sequence of beta-galactosidase XI; Peptide ordering procedures and the complete sequence. J. Biol. Chem. 253, 5521, (1978)